Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Aug 16;13:907980. doi: 10.3389/fphar.2022.907980

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2022 Ding, Zhao, Wang, Li, Chen, Yong, Zhu, Tian, Liu, Yu, Zhou, Gu, Yao and Gao.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Abelmoschus manihot L. flower (TEA) can alleviate uric acid (UA)-induced NRK-52E cell injury. (A) Effect of UA and TEA on morphological changes. NRK-52E cells were treated with UA and TEA for 24 h. Cell morphology was analyzed using phase-contrast microscopy (magnification, ×100). (B) Effects of TEA on cell viability. NRK-52E cells in 96-well plates were treated with TEA and UA for 24 h. Cell viability was evaluated using a CCK-8 assay. Data are expressed as the percentages of living cells versus the control group (CONT, 0 μg/ml) (means ± SD, n = 3 in each group). ∗∗∗p < 0.001 versus the CONT group; or ###p < 0.001 versus UA group in CONT. (C) Living and dead cells were stained with calcein-AM or PI and then visualized by inverted fluorescence microscopy (magnification, ×200). The cells emitting green fluorescence were living cells, and those emitting red fluorescence were dead cells.