Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Aug 16;13:907980. doi: 10.3389/fphar.2022.907980

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2022 Ding, Zhao, Wang, Li, Chen, Yong, Zhu, Tian, Liu, Yu, Zhou, Gu, Yao and Gao.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Abelmoschus manihot L. flower (TEA) attenuates uric acid (UA)-induced pyroptosis by inhibiting the expression of GSDME-NT, NLRP3, and caspases. (A) Effects of TEA on ERK1/2 phosphorylation and NLRP3 expression induced by UA. NRK-52E cells in 6-well plates were treated with TEA and UA for 24 h. (B) Statistical analyses of ERK1/2 phosphorylation and NLRP3 expression (means ± SD, n = 3 in each group). All data were corrected with respect to the control group. ∗∗∗p < 0.001 versus the model group (UA in 640 μg/ml); ∗∗p < 0.01 versus the model group (UA in 640 μg/ml); ∗p < 0.05 versus the model group (UA in 640 μg/ml). (C) Effects of TEA on caspase-8 and GSDME expression induced by UA. NRK-52E cells in 6-well plates were treated with TEA and UA for 24 h. (D) Statistical analyses of caspase-8 and GSDME expression (means ± SD, n = 3 in each group). All data were corrected with respect to the control group. ∗∗∗p < 0.001 versus the model group (UA in 640 μg/ml); ∗∗p < 0.01 versus the model group (UA in 640 μg/ml); ∗p < 0.05 versus the model group (UA in 640 μg/ml). (E) Effects of TEA on caspase-3 expression induced by UA. NRK-52E cells in 6-well plates were treated with TEA and UA for 24 h. (F) Statistical analyses of caspase-3 expression (means ± SD, n = 3 in each group). All data were corrected with respect to the control group. ∗∗∗p < 0.001 versus the model group (UA in 640 μg/ml); ∗∗p < 0.01 versus the model group (UA in 640 μg/ml); ∗p < 0.05 versus the model group (UA in 640 μg/ml). (G) The LDH release rate of UA and TEA on cells. NRK-52E cells in 96-well plates were treated with UA and TEA for 24 h. LDH release rate was analyzed by LDH cytotoxicity Assay Kit. All data were corrected with respect to the control group (0 μg/ml) (means ± SD, n = 6 in each group). ∗∗∗p < 0.001 versus the model group (UA in 640 μg/ml).