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. 2022 Aug 16;9:943384. doi: 10.3389/fmolb.2022.943384

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Copyright © 2022 Huang, Dong, Zhang, Fu, Lv and Wu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Ephrin family genes are abnormally expressed in HCC cell lines and HCC tissues. (A–H) RT–qPCR analysis of the mRNA expression of ephrin genes in five HCC cell lines (HCC-LM3, MHCC97-H, SMMC 7721, Huh-7, and HepG2) and a normal liver cell line (LO2). EFNA1 (A), EFNA2 (B), EFNA3 (C), EFNA4 (D), EFNA5 (E), EFNB1 (F), EFNB2 (G), and EFNB3 (H). (I–P) The mRNA expression of ephrin genes in 40 pairs of HCC tissues and adjacent para-carcinoma tissues was evaluated using qPCR. EFNA1 (I), EFNA1 (I), EFNA2 (J), EFNA3 (K), EFNA4 (L), EFNA5 (M), EFNB1 (N), EFNB2 (O), and EFNB3 (P). GAPDH was used as an internal control. Error bars represent the means ± SEM (triplicate experiments). *p < 0.05; **p < 0.01; ***p < 0.001.