TABLE 1.
Summary of the cellular characteristics. Volume, area, CMF, MCH, Young’s modulus, plasticity, trapping time and recovery of cRBClipids are located between values of nRBC and nRET. Diameter, height and sphericity are, however, more extreme than in nRET. The increased sphericity of cRBC might be linked to the increased band 3 and F-actin expression.
| Cell type | nRBC | nRET | cRBClipids | cRBCw/o lipids |
|---|---|---|---|---|
| Variety of shapes | biconcave | lobular, humped, asymmetrical, biconcave | lobular, humped, asymmetrical, biconcave | spherical, lobular, humped |
| Diameter [µm] microscopy | 7.4 ± 0.32 | 8.2 ± 0.47 | 8,7 ± 0,35 | 9,4 ± 0,19 |
| Volume [µm 3 ] DHM | 110.7 ± 20.4 | 155.4 ± 33.8 | 125.9 ± 41.3 | 139.7 ± 53.1 |
| Height [µm] DHM | 2.21 ± 0.5 | 1.99 ± 0.5 | 1.75 ± 0.4 | 3.33 ± 0.7 |
| Area [µm 2 ] DHM | 56.7 ± 8.2 | 87.5 ± 23.5 | 82.1 ± 23.5 | 46.0 ± 14.8 |
| Sphericity DHM | 0.45 ± 0.2 | 0.42 ± 0.2 | 0.53 ± 0.4 | 1.11 ± 0.1 |
| CMF [nm] DHM | 30.7 ± 20.4 | 35.3 ± 17.9 | 32.5 ± 14.1 | 44.9 ± 19.2 |
| MCH [pg] DHM | 31.2 ± 11.2 | 38.1 ± 7.3 | 34.9 ± 13.4 | 33.5 ± 10.0 |
| Young’s modulus [kPa] AFM | 2.03 | 2.85 | 2.32 | 0.09 |
| Plasticity index AFM | 0.803 | 0.699 | 0.726 | 0.580 |
| Trapping time [s] OT | 0.61 | 1.25 | 0.75 | n.a. |
| Recovery time [s] OT | 10 | 12.5 | 8.5 | n.a. |