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. 2022 Aug 16;13:960909. doi: 10.3389/fimmu.2022.960909

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Copyright © 2022 Papait, Ragni, Cargnoni, Vertua, Romele, Masserdotti, Perucca Orfei, Signoroni, Magatti, Silini, De Girolamo and Parolini

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Flow cytometry analysis of hAMSC EV uptake by PBMC (A) PBMC were cultured without stimulation in presence or absence of 5 × 10⁹ EVs loaded with CFSE to evaluate uptake. A staining for the different immune subsets was performed to evaluate differences in the uptake capability. (B) Mean fluorescence analysis of the different immune subpopulations normalized on the negative fraction.