FIGURE 1.

The changes of lncRNA MEG3 expression in the spinal cord injury model in vivo. After the construction of the SCI mouse model using a modified Allen's weight drop apparatus, the spinal cord tissues were isolated. (A) Basso mouse Scale (BMS) score was applied to assess the motor function of mice. (B) Nissl staining was applied to analyze the pathological characteristics of spinal cord tissues in mice (scale bar: 5 μm). (C) GEO database (GSE45376) was applied to the expression profile of lncRNAs. (D) The level of lncRNA MEG3 in serum on days 1, 3, 7, 14, 21, and 28 after SCI has been detected by qRT‐PCR. (E) Quantitative real‐time PCR (qRT‐PCR) was performed to measure the expression of lncRNA MEG3 in the spinal cord tissues. (F) The levels of TNF‐A, IL‐1B, and IL‐6 in serum on days 1, 3, 7, 14, 21, and 28 after SCI has been detected by ELISA. Primary mouse microglia were isolated from the normal mouse and cultured. CD206 (M2) protein expression (G) and CD16/32 (M1) protein expression (H) was measured by flow cytometry assay. *P < 0.05 vs. Sham. SCI, spinal cord injury. Data are represented as the mean ± SD of three independent assays