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Pulse-chase experiment with WT At ProXp-ala and At Ala-tRNAPro. Deacylation of 100 nM WT At Ala-tRNAPro by 750 nM WT At ProXp-ala was measured without dilution (•) and after 100-fold dilution by 1.0 μM unlabeled, uncharged WT At tRNAPro at 0 s (
) or 60 s (
). Deacylation curves represent the average of three independent trails fit to a single exponential equation with the standard deviation indicated.
