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. 2022 Jul 12;298(9):102255. doi: 10.1016/j.jbc.2022.102255

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© 2022 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Bimolecular fluorescence complementation analysis to probe the interaction between ProXp-ala and two unrelated proteins using an A. thaliana protoplast transient expression system. A split nYFP domain was fused to the C terminus of FL, ΔCTD, and CTD ProXp-ala, while a split cYFP domain was fused to a cysteine protease (RD21A) and an isoform of protein phosphatase 2A (PP2A). (A–C) Coexpression of FL-, ΔCTD-, and CTD-nYFP with RD21A-cYFP. (D–F) Coexpression of FL-, ΔCTD-, and CTD-nYFP with PP2A-cYFP. Protein–protein interactions are indicated by a reconstituted YFP signal. The scale bar represents 20 μm.