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Electrophoresis in agarose gel was running to make evident the detection limit of the Cryptococcus neoformans/Cryptococcus gattii complex nested PCR. Lanes in gel were loaded with molecular weight marker (M) and amplicons from PCR reactions employing (1) 40 ng, (2) 400 pg, (3) 4 pg, (4) 40 fg, (5) 4 fg, (6) 2 fg, (7) 1 fg, (8) 0 fg of C. neoformans DNA.
