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DNA transformation in the presence of competing polyanions. Bacteria (either Opa+ or Opa−) were picked from HEPES-buffered (pH 8)-agarose-based plates and were transformed with 1-μg quantities of donor DNA in the presence of various amounts of either unmarked gonococcal chromosomal (GC) or herring sperm (Sperm) DNA (A) or sodium heparin (B). The data presented are mean transformation frequencies ± standard errors (n = 6 [A] or 12 [B]).
