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TABLE 1.

Transformation of N. gonorrhoeae expressing different Opa proteins

Opa phenotype Oa Transformation frequency ([no. of transformants/μg of DNA/CFU] × 103)
% Errord
Overallb Ma Subpopulationc
Pa
Opa+ Opa
Opa NAe 0.77 NA NA NA NA NA
OpaA 0.88 2.83 0.18 7.45 1.88 0.47 2
OpaF 0.93 3.35 0.16 7.25 2.61 0.35 <1
OpaH 0.54 1.86 0.08 2.73 1.73 0.13 3
OpaI 0.79 2.26 0.16 3.65 1.88 0.27 <1
a

O, M, and P values refer to the fraction of Opa+ organisms in the initial, final, and transformant populations, respectively. 

b

Overall frequency represents the mean value (n = 5) determined without phenotypic evaluation of the culture. 

c

Subpopulation frequencies were obtained for each Opa+ and Opa subpopulation within a culture as described in Materials and Methods. 

d

% Error was calculated as indicated in Materials and Methods. 

e

NA, not applicable.