TABLE 2.
Effect of medium composition and DNase I treatment on transformation
| Opa phenotype | DNase I treatmenta | Values for organisms grown on:
|
|||
|---|---|---|---|---|---|
| Agar-based medium
|
Agarose-based medium
|
||||
| Transformation frequencyb | Ratioc | Transformation frequencyb | Ratioc | ||
| Opa− | − | 5.19 ± 0.82 | 0.063 | 9.52 ± 1.97 | 0.047 |
| + | 0.33 ± 0.09 | 0.45 ± 0.08 | |||
| OpaA | − | 54.9 ± 1.54 | 0.018 | 161.0 ± 2.87 | 0.045 |
| + | 0.99 ± 0.28 | 7.28 ± 1.28 | |||
a
± 1 U of DNase I.
b
Calculated with the following formula: (no. of transformants/μg of DNA/CFU) × 103. Values are means ± standard errors for data from 15 experiments.
c
Mean frequency in the presence of DNase I/mean frequency in the absence of DNase I.