TABLE 3.
Effect of different pilins on transformation under low-density conditions
| Expt | Opa phenotype | Values for organism with pilin encoded by:
|
|||||
|---|---|---|---|---|---|---|---|
|
pilE7302
|
pilE6
|
pilE3
|
|||||
| Transformation frequencya | Ratio (P)b | Frequencya | Ratio (P)b | Frequencya | Ratio (P)b | ||
| 1 | Opa− | 0.99 ± 0.13 | 18.9 (<0.02) | 0.71 ± 0.13 | 4.94 (<0.01) | 1.75 ± 0.63 | 1.7 (NS) |
| OpaA | 18.8 ± 5.25 | 3.55 ± 0.67 | 2.98 ± 0.86 | ||||
| 2 | Opa− | 0.92 ± 0.23 | 4.68 (<0.05) | 0.67 ± 0.14 | 7.17 (NS) | 1.22 ± 0.17 | 4.03 (<0.02) |
| OpaI | 4.33 ± 1.06 | 4.84 ± 2.01 | 4.92 ± 0.90 | ||||
a
Calculated as follows: (no. of transformants/μg of DNA/CFU) × 103. Values are means ± standard errors for data from five experiments.
b
Mean frequency for Opa+ organisms/mean frequency for Opa− organisms. P values indicate the degree of significance between the reported Opa+ and Opa− transformation frequencies. NS, not significant at a P value of 0.05.