Figure 1.
Aerobic metabolism and lactic fermentation in OHSU-974-FAcorr and OHSU-974-S91 cells. (A) Oxygen consumption rate (OCR). (B) Aerobic ATP synthesis through FoF1 ATP-synthase. For both graphs, data were obtained using pyruvate plus malate (grey columns) or succinate (white columns) as respiring substrates. (C) P/O value, an indicator of OxPhos efficiency, is calculated as the ratio between the synthesized ATP and the OCR. (D) Lactate dehydrogenase (LDH) activity. (E) Glucose consumption. (F) Lactate release in the growth medium. (G) Lactic fermentation yield. (H) Mitochondrial respiratory complexes activity. Data are reported as mean ± SD, and each graph is representative of at least 3 independent experiments. Statistical significance was tested opportunely with the unpaired t-test or two-way ANOVA. *, ***, and **** represent a p < 0.05, 0.001, and 0.0001, respectively, between OHSU-974-S91 cells and the OHSU-974-FAcorr cells, used as control.