Figure 5.

Expression evaluation of proteins involved in mitochondrial dynamics in OHSU-974-FAcorr and OHSU-974-S91 mitochondrial dynamics. (A) Western blot (WB) signals of CLUH (a regulator of mitochondria dynamic), DRP1 (a marker of mitochondrial fission), MFN2, OPA1 (two markers of mitochondrial fusion), and β-Actin. Each WB signal is representative of at least 3 independent experiments. (B) Densitometric analysis of the WB signals reported in Panel A, normalized versus β-Actin signal. Data are reported as mean ± SD, and each graph is representative of at least 3 independent experiments. Statistical significance was tested with an unpaired t-test. ** represents a p < 0.01 between OHSU-974-S91 cells and the OHSU-974-FAcorr cells used as control.