Table 2.
Specificity of quantitative PCR.
| Species (n = 10) | CT value |
|---|---|
| Mycobacterium leprae | 12.5 ± 0.5 |
| Mycobacterium tuberculosis | >40 |
| Mycobacterium avium | >40 |
| Mycobacterium fortuitum | >40 |
| Mycobacterium smegmatis | >40 |
| Mycobacterium intracellulare | >40 |
| Mycobacterium chimera | >40 |
| Mycobacterium nonchromogenicum | >40 |
| Mycobacterium colombiense | >40 |
| Mycobacterium abscessus subspboletti | >40 |
| Other dermatological diseases (n = 10) | |
| Pemphigus foliaceus (n = 2) | >40 |
| Pemphigus vulgaris (n = 1) | >40 |
| Nummular eczema (n = 1) | >40 |
| Lichenified eczema (n = 1) | >40 |
| Porphyria (n = 2) | >40 |
| Lupus erythematosus (n = 3) | >40 |
| Healthy individuals (n = 10) | |
| Normal skin | >40 |
qPCR, quantitative PCR; CT, cycle threshold.
Specificity of the primers relative to other species of mycobacteria, dermatological diseases (non leprosy) and healthy individuals.