Table 4.
No. of isolates | SCCmec typing |
Resistance profilea |
Biofilm productionb |
||||||
---|---|---|---|---|---|---|---|---|---|
mec class | ccr type | SCCmec | CLI | TET | LZD | ERY | Glucose | NaCl | |
8 | C2 | 5 | V | 7 (88) | 3 (38) | 0 (0) | 7 (88) | 7 (88) | 4 (50) |
1 | C2 | 1 + 2 + 5 | Variant | 0 (0) | 0 (0) | 0 (0) | 0 (0) | 1 (100) | 0 (0) |
3 | C2 | 1 + 4 + 5 | Variant | 3 (100) | 3 (100) | 0 (0) | 3 (100) | 3 (100) | 2 (67) |
12 | NT | 4 | NT | 10 (83) | 3 (25) | 0 (0) | 11 (92) | 8 (67) | 5 (42) |
1 | NT | 5 | NT | 1 (100) | 1 (100) | 0 (0) | 1 (100) | 1 (100) | 0 (0) |
30 | NT | NT | NT | 30 (100) | 3 (10) | 1 (3) | 30 (100) | 23 (77) | 18 (60) |
NT, not typeable; CLI, clindamycin; TET, tetracycline; LZD, linezolid; ERY, erythromycin.
Methicillin-resistance was evaluated using the cefoxitin disk test, minimum inhibitory concentration was determined using the broth microdilution method. Biofilm formation was evaluated by crystal violet staining. Amplification of the ica operon and SCCmec typing were performed by multiplex PCR.
All isolates were resistant to cefoxitin, oxacillin, norfloxacin, levofloxacin and trimethoprim/sulfamethoxazole. All isolates were susceptible to vancomycin.
Strong producers.