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Influence of tra boxes II and III on expression of traI. Overnight cultures of the test strains were diluted into and incubated in fresh ABM medium supplemented with synthetic AAI (40 nM). Samples were removed at the indicated times and assayed for β-galactosidase activity as described in Materials and Methods. The experiment was repeated twice. Bars show the standard deviation for each data point. ▵, NT1(pKP19, pSVB33); ●, NT1(pTB12, pSVB33); ◊, NT1(pKP19); ■, NT1(pTB12).
