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. 2017 Nov 13;22(1):47–50. doi: 10.1016/j.bjid.2017.10.002

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© 2017 Sociedade Brasileira de Infectologia. Published by Elsevier Editora Ltda.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. 1 — Multiplex PCR assay for simultaneous detection of 16S rRNA (∼900 bp), as an amplification control, and carbapenemase genes. BlaKPC: Lane 1, K. pneumonia IOC4955 (positive control); Lane 2, K. pneumonia ATCC 700603 (negative control); Lane 3, 5843; Lane 4, 5939; Lane 5 5958. BlaNDM: Lane 1, E. cloacae CCBH10892 (positive control); Lane 2, K. pneumonia ATCC 700603 (negative control); Lane 3, 5843; Lane 4, 5939; Lane 5 5958. BlaOXA-48: Lane 1, K. pneumoniae CCBH9976 (positive control); Lane 2, K. pneumonia ATCC 700603 (negative control); Lane 3, 5843; Lane 4, 5939; Lane 5 5958. BlaVIM: Lane 1, P. aeruginosa CCBH11808 (positive control); Lane 2, K. pneumonia ATCC 700603 (negative control); Lane 3, 5843; Lane 4, 5939; Lane 5 5958. BlaIMP: Lane 1, K. pneumonia BR01 (positive control); Lane 2, K. pneumonia ATCC 700603 (negative control); Lane 3, 5843; Lane 4, 5939; Lane 5 5958. Lane M, 100-bp DNA ladder (Invitrogen). The electrophoresis was run in a 2% agarose gel, which was stained with ethidium bromide.