. 2017 Nov 13;22(1):47–50. doi: 10.1016/j.bjid.2017.10.002

Table 1.

The multiplex PCR (carbapenemase and 16S genes) by using specific oligonucleotide primers.

Gene	Primer	Product (pb)	Reference	Positive controls
16S	U3	∼900	James G et al., 2010¹⁰	–
16S	U4	∼900	James G et al., 2010¹⁰	–
bla_KPC	KPC-F	1011	Yigit H et al., 2001¹¹	K. pneumoniae IOC4955
bla_KPC	KPC-R	1011	Yigit H et al., 2001¹¹	K. pneumoniae IOC4955
bla_NDM	NDM F	512	GenBank^a KP772192.1	E. cloacae CCBH10892
bla_NDM	NDM R	512	GenBank^a KP772192.1	E. cloacae CCBH10892
bla_OXA-48	OXA F	440	GenBank^a KP849468.1	K. pneumoniae CCBH9976
bla_OXA-48	OXA R1	440	GenBank^a KP849468.1	K. pneumoniae CCBH9976
bla_VIM	VIM F	332	GenBank^a KR259332.1	P. aeruginosa CCBH11808
bla_VIM	VIM R2	332	GenBank^a KR259332.1	P. aeruginosa CCBH11808
blaIMP	IMP F2	440	GenBank^a KF745070.2	K. pneumonia BR01
blaIMP	IMP R	440	GenBank^a KF745070.2	K. pneumonia BR01

Accession number of the sequence used for primer design.