. 2019 Apr 24;23(2):102–110. doi: 10.1016/j.bjid.2019.03.002

Table 1.

Primer sequences and melting temperatures for identification of β-lactamases.

Primers	Gene	Sequence 5′–3′	Tm (°C)
SHV_Fw	bla_SHVlike	AGCCGCTTGAGCAAATTAAA	60
SHV_Rv	bla_SHVlike	TCCCGCAGATAAATCACCAC	60
TEM_Fw	bla_TEMlike	GCCTTCCTGTTTTTGCTCAC	60
TEM_Rv	bla_TEMlike	GATACGGGAGGGCTTACCAT	60
CTXM1_Fw	bla_CTX-M-1group	TTAGGAARTGTGCCGCTGT	59
CTXM1_Rv	bla_CTX-M-1group	TACAAACCGTTGGTGACGA	59
CTXM2_Fw	bla_CTX-M-2group	GACGCTACCCCTGCTATTTA	58
CTXM2_Rv	bla_CTX-M-2group	AGAAACCGTGGGTTACGATT	58
CTXM8_Fw	bla_CTX-M-8group	TCGCGTTAAGCGGATGATGCT	64
CTXM8_Rv	bla_CTX-M-8group	TCGGTGACGATTTTCGCGGCA	64
CTXM9_Fw	bla_CTX-M-9group	TGGTGACAAAGAGARTGCAA	58
CTXM9_Rv	bla_CTX-M-9group	GATTCTCGCCGCTGAAG	58
CTXM25_Fw	bla_CTX-M-25group	CGACAGCCTGTGTTTCGCTGC	64
CTXM25_Rv	bla_CTX-M-25group	TCGGTGACWATTCTGGCGGCA	64
VEB_Fw	bla_VEBlike	TTCAAATGCTCAARCTGACAA	58
VEB_Rv	bla_VEBlike	TCCACGTTATTTTTGCAATG	58
GES1_Fw	bla_GES-1like	CGCTTCATTCACGCACTATT	60
GES1_Rv	bla_GES-1like	CGTGCTCAGGATGAGTTGTG	60
GES10_Fw	bla_GES-10like	GAGAAGCTAGAGCGCGAAAA	60
GES10_Rv	bla_GES-10like	ACTTGACCGACAGAGGCAAC	60
PER1_Fw	bla_PER-1like	ACTGTAGGCGTTGCAGTGTG	60
PER1_Rv	bla_PER-1like	TAATTTGGGCTTAGGGCAGA	60
PER2_Fw	bla_PER-2like	GTTCTGCATCAGGTCGATCA	60
PER2_Rv	bla_PER-2like	CCATCAGGCAACATAATGACG	60