FIG. 1.

Run-off transcription analysis. (A) A representative run-off transcription time course experiment is shown for Eς⁷⁰ and ς⁷⁰ mutants at 37°C. Each experiment was repeated at least five times, and the error was less than 15%. (B) Abortive transcription by Eς⁷⁰D61A at 37°C. Synthesis of the three nucleotide abortive RNA transcripts (ApUpG) from λ p_R is shown relative to the wild type. The holoenzyme concentration was 0.008 μM and the template concentration was 1.5 nM. (C) Run-off transcription as a function of temperature. A comparison of run-off transcription activities at 60 min at 25 and 42°C is shown. Activities are normalized to the wild type, and the error indicated was less than 15%. WT, wild type.