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. 2022 Aug 30;22:936. doi: 10.1186/s12885-022-10033-4

Fig. 5.

Fig. 5

Src overexpression alters cell shape of plectin knockout melanoma cells. A Src was overexpressed in B16 and PKO1. Expression and phosphorylation of PYK2 was determined by western blotting analysis. B Cells were immunostained with anti-Flag/Alexa fluor 488, Rhodamine-phalloidin and DAPI 1 d after Src introduction. Cell shapes are outlined with a dotted line in the bright-field image. Scale bar = 50 μm. C The number of elongated cells were quantified (n = 5). *; p < 0.05 vs control Src(−), #; p < 0.05 vs PKO1 Src(−), ns; no significance vs control Src(−)