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. 2022 May 24;6(9):2474–2487. doi: 10.1002/hep4.2014

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© 2022 The Authors. Hepatology Communications published by Wiley Periodicals LLC on behalf of American Association for the Study of Liver Diseases.

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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HBV‐CRISPR exerts an antiviral effect accompanied by a significant reduction in cccDNA in HBV‐infected primary human hepatocytes (PHHs). PHHs isolated from humanized liver chimeric mice were lentivirally transduced with tandem Cas9 gRNA and HBV gRNA (or NC gRNA)–expressing vectors (10 multiplicity of infection [MOI]) 19 days after HBV inoculation (500 GEq/cell). (A) Experimental protocol. (B) cccDNA levels, intracellular pgRNA levels, and supernatant HBV DNA, HBs antigen, and HBe antigen levels in PHHs 35 days after HBV inoculation (n = 4; *p < 0.05, **p < 0.01).