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Identification of circulating B cells specific for ECL2 and ECL4 of TP0856 and TP0858. IgG+ B cells specific for ECL2 and ECL4 in TP0856 (A) and TP0858 (B) were identified within the IgG+ PfTrxNeg gate as cells double positive for SP-AF405 and SP-AF647; PBMCs from a normal rabbit were used as a control. The mean frequencies of the individual ECL-specific IgG+ B cells are shown in the accompanying bar graphs.
