FIG 3.
Host-specific response to microbiome reprogramming. (A) Number of significantly different host proteins identified (Student’s t test, P < 0.05; FDR = 0.05; S0 = 1) across different conditions; (B) STRING analysis for host proteins with significant changes in abundance across different conditions. Immediate and established infection C. neoformans samples were grouped, and immediate and established infection C. neoformans and K. pneumoniae samples were grouped. Labels represent themes observed within designated clusters based on gene names and protein functions provided in STRING. (C) 1D annotation enrichment for categories of proteins based on keywords. Student's t test, P < 0.05; FDR = 0.05. (D) Mean LFQ intensity of all immune- and inflammation-associated proteins (as designated by GOBP terms) from the host proteome. (E) Measurement of LFQ intensity of IL-1β and TNF-α from proteome data across conditions. Statistical analysis using Student's t test: *, P < 0.05; **, P < 0.001; ***, P < 0.0001. (F) Western blot for 1L-1β and TNF-α across samples and loading control SDS-PAGE. The experiment was performed in biological quadruplicate and technical duplicate.