FIG 7.
Enhanced bacterial protein response upon established coinfection. (A) Volcano plot for comparison of bacterial proteins between established C. neoformans plus immediate K. pneumoniae coinfection and established C. neoformans plus established K. pneumoniae coinfection. The Venn diagram depicts the number of significantly different proteins identified under each condition. (B) Distribution of GOBP terms between bacterial proteins with significant increases or decreases in abundance upon coinfection. (C) PCA plot of the experiment. (D) Heat map of normalized LFQ intensities by Euclidean distance for K. pneumoniae proteins identified under the specified conditions. Volcano plots: Student’s t test, P < 0.05; FDR = 0.05; S0 = 1. Green denotes K. pneumoniae proteins. The experiment was performed in biological quadruplicate. Letters a to e designate a protein cluster for reference as described within the main text.