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. 2022 Jul 19;13(4):e01247-22. doi: 10.1128/mbio.01247-22

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Copyright © 2022 Laczkovich et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 3 — Identification of two novel sORFs found near the uncharacterized transcriptional regulator Rgg1518. (A) Ribosome footprint density profiles of rio83 and rio84 found near spv_1518 (Rgg1518). Blue arrows represent sORFs identified by Ribo-RET, and gray arrows represent previously annotated ORFs. (B) Volcano plot of wild-type D39 versus Δrgg1518 transcript fold changes. Genes of interest with the highest fold change differences are indicated on the graph. (C) qRT-PCR validation of spv_1517 expression in wild-type (WT) D39 versus the Δrgg1518 mutant.