FIG 5.

(A) Airyscan confocal analysis of IFA of CDC50C-HA:loxP trophozoites costained with EXP2 (PF3D7_1471100), an exported PV protein; ERD2, a Golgi marker (PF3D7_1353600); and PMV, an ER marker (PF3D7_1323500). Scale bar, 2 μm. (B) Flow cytometry analysis of fluorescent lipid uptake in live WT (DMSO) and CDC50C null (RAP) trophozoites labeled at 36 h postinvasion. Histograms are overlaid, each representing 10,000 cells for each treatment. Cells were gated for DNA content and for green fluorescence. No detectable shift in histogram curves was seen for each lipid in RAP-treated samples. Data are representative of one of three independent experiments, each of which showed the same outcome. Control samples, with no lipid added, were analyzed to validate the gating protocol for lipid signal. Right panel, examples of the stained cells visualized by fluorescence microscopy. Scale bar, 2 μm.