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. 2022 Jul 12;13(4):e01635-22. doi: 10.1128/mbio.01635-22

TABLE 1.

Oligonucleotide primers used in this study

Primer Name Sequence
1 CDC50A 5HR F GCGGCCGCAGATCTCTCGAGCGATATTGGACACCAAATTGTTTA
2 CDC50A 5HR R CGAAGTTATTGTATATTATTTTTTTTATTTACCTACATGTGATTTATGTAATTCCATTTC
3 CDC50A reco F GTATATATATATATATTTATATATTTTATATTCTTTTAGATTTCTGGCTCATGAACGAAAAGTACAAGAACGCATTAAACATG
4 CDC50A reco R CAGATCCGCCTGAACCGGATCCCAAGAAGAAGAAGATGTAAATATAAG
5 CDC50B 5HR F GCGGCCGCAGATCTCTCGAGTGAGTAATCTTAAAAATGACATGTTTATATC
6 CDC50B 5HR R CGAAGTTATTGTATATTATTTTTTTTATTTACCTTTATATAATTGTACATTTTGAGGTG
7 CDC50B reco F GTATATATATATATATTTATATATTTTATATTCTTTTAGATCACATTTACTTTTGGATGGAGC
8 CDC50B reco R CAGATCCGCCTGAACCGGATCCCTTGTTCTCGTACTTGTTCATC
9 CDC50C reco F GTATATATATATATATTTATATATTTTATATTCTTTTAGATGAGTGGAACGCTAAGAAAAGTTTC
10 CDC50C reco R CCGCCTGAACCGGATCCATGCAATGGACAGAAACACAACTTC
11 CDC50C 5HR F GATCTCTCGAGCCAGAGTACGAATTCATGAATGCTTTTAAACAACAAG
12 CDC50C 5HR R CGAAGTTATTGTATATTATTTTTTTTATTTACCTGCTGGCCATACGTTTTGAAG
13 5HR seq F CAGCTATGACCATGATTACGCC
14 Reco seq F CATTATACGAAGTTATTATATATGTATATATATATATATTTATATATTTTATATTC
16 PKAc screen F GAAGGACAGTGATTCTAGTGAACAG
17 PKAc WT screen R CAATTTCTTCATCAAATGTTTGCAATTGTTATC
18 HA R GCATAGTCAGGAACATCGTAAGG
19 Exi R CCGTTCAAATCTTCTTCAGAAATCAAC
20 3′ int F CAGCTATGACCATGATTACGCC
21 CDC50A 5′ int F CTTTAGATTATGATGATAATTTTTTGGAAGAAAAG
22 CDC50A WT R GTGTATATTTAAAAATCAGGATTTTACTATATCCTC
23 CDC50B 5′ int F CAGTTATGTGTCTTCCCTTTGTATTATTTTG
24 CDC50B WT R CTTTTGGTTATTAAATGTGTATCGAAATAATAC
25 CDC50C 5′ int F GTCGCAGTTCATGGGAAGG
26 CDC50C WT R GGGAATGGTCTGCTCCTGCT
27 T2A BSD amp F GCGGCATGGACGAGCTGTACAAGAGTGGAGAAGGAAGAGG
28 T2A BSD amp R GTGTTGATGGTTTTGGGCTAGCTTAGCCCTCCCACACATAACC
29 Screen T2A F CGAGGACTACACCATCGTGG
30 GCtag WT F CTAAGAATATTCATTCCTACGATG
31 GCtag 3′ int F CAATGGCACCTTTGTCTCAAG
32 GCtag 5′ int F CATGGGCAAATGGTGTAGATG
33 GCtag 5′ int R CCTCCATGTGCACCTTG
34 GCtag WT R CGAATGTTCGGAAAAATATTCATGTGC
35 p230p Int screen F CTATATGGTATCCAAAACCTTTAAATTATATAGC
36 p230p WT screen R GAGGAATTTTTAAATATGATATACCTTTATCATTAG