TABLE 1.
LC gradient
| Time (min) | Flow (mL/min) | % mobile phase B | Curve |
|---|---|---|---|
| 0.000 | Run | ||
| 0.000 | 0.500 | 2.0 | 5 |
| 1.000 | 0.500 | 2.0 | 5 |
| 2.500 | 0.500 | 98.0 | 5 |
| 4.500 | 0.500 | 98.0 | 5 |
| 5.500 | 0.500 | 2.0 | 5 |
| 7.500 | 0.500 | 2.0 | 5 |
| 7.500 | Stop run | ||
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