TABLE 2.
Q Exactive Plus (Thermo Scientific) instrument parameters
| Parameter | Value |
|---|---|
| Runtime | 0 to 7.5 min |
| Polarity | Positive |
| Default charge state | 1 |
| Full MS | |
| Resolution | 70,000 |
| AGC targeta | 3e6 |
| Maximum IT | 246 ms |
| Scan range | 100 to 1500 m/z |
| dd-MS2/dd-SIM | |
| Resolution | 17,500 |
| AGC target | 1e5 |
| Maximum IT | 54 ms |
| Loop count | 5 |
| TopN | 5 |
| Isolation window | 1.0 m/z |
| (N)CE/stepped (N)CE | NCE: 20, 40, 60 |
| dd Settings | |
| Minimum AGC target | 8.00e3 |
| Intensity threshold | 1.5e5 |
| Peptide match | Preferred |
| Exclude isotopes | On |
| Dynamic exclusion | 10.0s |
| Tune data | |
| Spray voltage (+) | 3800.00 |
| Capillary temp (+ or +–) | 320.00 |
| Sheath gas (+ or +–) | 35.00 |
| Aux gas (+ or +–) | 10.00 |
| Spare gas (+ or +–) | 0.00 |
| Max spray current (+) | 100.00 |
| Probe heater temp (+ or +–) | 0.00 |
| S-lens RF level | 50.00 |
| Ion source | HESI |
a
AGC, Automatic Gain Control; IT, injection time; dd-MS2/dd-SIM, data-dependent MS2/data-dependent selected ion monitoring; NCE, normalized collision energy; RF, radiofrequency.