TABLE 1.
The genotypes and anti-complement phenotypes of B. burgdorferi strains used in this studya
| Strains | Genotype |
Geographical location | Source | Anti-complement phenotypesb |
Accession no. of genome | References of source | |||
|---|---|---|---|---|---|---|---|---|---|
| MLST | RST | OspC | |||||||
| Rodentc | Quail | ||||||||
| B31Ad | 1 | 1 | A | North America (New York, USA) | Tick (I. scapularis) | − | − | n.a. | (92) |
| B313e | 1 | 1 | A | North America (New York, USA) | Tick (I. scapularis) | − | − | n.a. | (93) |
| B31-reff | 1 | 1 | A | North America (New York, USA) | Tick (I. scapularis) | + | + | GCA_000008685.2 | (74, 94) |
| B31-5A4 | 1 | 1 | A | North America (New York, USA) | Tick (I. scapularis) | + | + | CP094597-CP094617 | (74, 94) |
| PAbe | 1 | 1 | A | Europe (Germany) | Human (CSF) | + | + | GCA_002151485.1 | (93) |
| PAli | 1 | 1 | A | Europe (Germany) | Human (skin) | + | + | GCA_002151465.1 | (95) |
| BL206 | 1 | 1 | A | North America (New York, USA) | Human (blood) | + | + | JALGSN010000000 | (56) |
| ZS7 | 20 | 1 | B | Europe (Germany) | Tick (I. ricinus) | + | + | GCA_000021405.1 | (96) |
| PMeh | 20 | 1 | B | Europe (Germany) | Human (joints) | + | + | SRX828288 | (96) |
| 297 | 3 | 2 | K | North America (Connecticut, USA) | Human (CSF) | + | − | JALGSQ010000000 | (97, 98) |
| B379 | 3 | 2 | K | North America (New York, USA) | Human (skin) | − | + | CP094579-CP094596 | (99) |
| Bbss62 (Bbss62.h.ks) | 4 | 2 | H | North America (Massachusetts, USA) | Human (skin) | + | + | SAMN29767977 | This study |
| WI91-23 | 228 | 3 | I | North America (Wisconsin, USA) | Song Sparrow | − | + | GCA_000181855.2 | (100) |
| B408 | 16 | 3 | I | North America (New York, USA) | Human (skin) | + | − | CP094561-CP094578 | (99) |
| B331 | 16 | 3 | I | North America (New York, USA) | Human (skin) | + | − | GCA_002442595.1 | (99) |
| 29805 | 12 | 3 | M | North America (Connecticut, USA) | Tick (I. scapularis) | − | + | GCA_000172295.2 | (100) |
| B356 | 12 | 3 | M | North America (New York, USA) | Human (skin) | − | + | JALGSP010000000 | (101, 102) |
| N40-D10/E9 | 12 | 3 | M | North America (New York, USA) | Tick (I. scapularis) | + | + | JALGSO010000000 | (103) |
| CA11.2A | 333 | 3 | D | North America (California, USA) | Tick (I. pacificus) | + | + | GCA_000172315.2 | (104) |
| MM1 | 328 | 3 | U | North America (Minnesota, USA) | WF mouse | + | − | GCA_003367295.1 | (105) |
| JD1 | 11 | 3 | C | North America (Massachusetts, USA) | Tick (I. scapularis) | + | − | GCA_000166655.2 | (106) |
| cN40 | 19 | 3 | E | North America (New York, USA) | Tick (I. scapularis) | + | + | GCA_000166635.2 | (107) |
CSF, Cerebrospinal fluid. MLST, Multilocus sequence type. RST, ribosomal RNA intergenic space type. Not available because these strains were not included in the phylogenetic analysis. WF, white-footed.
The results were derived from Fig. 1. +, defined by the strains with greater than the threshold values (recruited significantly greater levels of mouse C5b-9 or quail C8 on the surface in the presence of indicated sera than B31-5A4 and had survival percentage greater than 50% in white-footed mouse or quail sera). Otherwise, those strains are shown as “- ” to indicate anti-complement phenotypes.
Mouse sera for complement deposition assays and white-footed mouse sera for serum resistance assays.
High-passage B. burgdorferi B31 missing lp21, lp25, lp28-1, lp28-3, lp28-4, lp36, cp9, cp32-6, cp32-8.
High-passage B. burgdorferi B31 missing lp5, lp17, lp21, lp25, lp28-1, lp28-2, lp28-3, lp28-4, lp36, lp38, lp54, lp56, cp9, cp32-4, cp32-6, cp32-8, cp32-9.
Reference strain of B. burgdorferi obtained from ATCC (ATCC 35210).