FIG 4.

Evaluating the binning strategies on the HMP data set. (A) Total number of bins reconstructed per strategy. Colors represent the MAG qualities, estimated with CheckM. (B) Proportion of MAGs reconstructed for each strategy, after common dereplication of the four strategies, at the species resolution (95% identity) or at the strain resolution (99% identity). Number of dereplicated MAGs from each strategy is compared to the number of maximum expected MAGs, which is the number of MAGs obtained after dereplication of all the four strategies together. (C, D): Number of reconstructed MAGs after independent dereplication using dRep for each binning strategy, at (C) species resolution, consisting of a 95% ANI score dereplication; and (D) strain resolution, consisting of a 99% ANI score dereplication. SASB, single-assembly single binning; SACB, single-assembly cobinning; CASB, coassembly single binning; CACB, coassembly cobinning; HQ, high quality; MQ, medium quality; LQ, low quality.