TABLE 2.
Effector | Sll0726 |
Slr1334 |
Catalytic efficiency ratio (%) (Slr1334/Sll0726) | ||||
---|---|---|---|---|---|---|---|
Km (mM) | Kcat (s−1) | Kcat/Km (M−1s−1) | Km (mM) | Kcat (s−1) | Kcat/Km (M−1s−1) | ||
No effector | 3.27 ± 0.21 | 138 ± 3.7 | 42.2 × 103 | 3.31 ± 0.14 | 7.5 ± 0.1 | 2.3 × 103 | 5.5 |
Glc-1,6-BP | 0.19 ± 0.04 | 415 ± 30.6 | 3038.9 × 103 | 0.09 ± 0.02 | 14.4 ± 0.7 | 158.1 × 103 | 5.2 |
Frc-1,6-BP | 26.86 ± 3.35 | 218 ± 21.3 | 8.1 × 103 | 0.03 ± 0.01 | 3.6 ± 0.2 | 117.5 × 103 | 1,450 |
a Km and Kcat values are means of triplicates with ± SD. Catalytic efficiency ratio is given in percentage and was calculated by the ratio of Kcat/Km of Slr1334 and Sll0726.