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. 2022 Jul 20;13(4):e01469-22. doi: 10.1128/mbio.01469-22

TABLE 2.

Kinetic parameters for the conversion of Glc-1P to Glc-6P by Sll0726 and Slr1334 in the presence of different effector molecules as indicateda

Effector Sll0726
Slr1334
Catalytic efficiency ratio (%) (Slr1334/Sll0726)
Km (mM) Kcat (s−1) Kcat/Km (M−1s−1) Km (mM) Kcat (s−1) Kcat/Km (M−1s−1)
No effector 3.27 ± 0.21 138 ± 3.7 42.2 × 103 3.31 ± 0.14 7.5 ± 0.1 2.3 × 103 5.5
Glc-1,6-BP 0.19 ± 0.04 415 ± 30.6 3038.9 × 103 0.09 ± 0.02 14.4 ± 0.7 158.1 × 103 5.2
Frc-1,6-BP 26.86 ± 3.35 218 ± 21.3 8.1 × 103 0.03 ± 0.01 3.6 ± 0.2 117.5 × 103 1,450

a Km and Kcat values are means of triplicates with ± SD. Catalytic efficiency ratio is given in percentage and was calculated by the ratio of Kcat/Km of Slr1334 and Sll0726.