FIG. 2.

Low CoA levels result in thiamine auxotrophy in a purF background. Growth curves were performed as described previously (24). Cultures depicted in panel A were grown at 30°C; those in panel B were grown at 34°C. Squares represent strain DM5342 [purF2085 zii-8038::Tn10d(Tc) coaA1(Ts)]; circles represent strain DM5343 [purF2085 zii-8038::Tn10d(Tc)]. Strains were grown in medium with gluconate as the sole carbon and energy source plus adenine in the absence (open symbols) or presence (solid symbols) of exogenous thiamine.