TABLE 1.
TPP and CoA pools in wild-type and panE mutant strains
| Strain | Addition(s)a | Level (avg ± SD) ofb:
|
Growth rate (μ)c | |
|---|---|---|---|---|
| TPP | CoA | |||
| Wild type (LT2) | ||||
| 1 | None | 62.4 ± 22.4 | 0.189 ± .003 | 0.48 ± 0.0 |
| 2 | Thi | NA | 0.186 ± .005 | 0.48 ± 0.01 |
| 3 | Pan | 53.8 ± 1.0 | 0.211 ± .023 | 0.46 ± 0.01 |
| 4 | Ade | 38.7 ± 13.0 | 0.149 ± .017 | 0.49 ± 0.01 |
| 5 | Ade-Thi | NA | 0.148 ± .002 | 0.46 ± 0.02 |
| 6 | Ade-Pan | 43.2 ± 15.1 | 0.240 ± .024 | 0.47 ± 0.01 |
| panE1::MudJ (DM63) | ||||
| 7 | None | 58.0 ± 13.6 | 0.023 ± .004 | 0.45 ± 0.04 |
| 8 | Thi | NA | 0.044 ± .017 | 0.48 ± 0.01 |
| 9 | Pan | 44.9 ± 9.8 | 0.164 ± .014 | 0.44 ± 0.02 |
| 10 | Ade | 14.5 ± 3.6 | 0.032 ± .011 | 0.25 ± 0.01 |
| 11 | Ade-Thi | NA | 0.023 ± .005 | 0.43 ± 0.05 |
| 12 | Ade-Pan | 40.1 ± 10.0 | 0.166 ± .016 | 0.49 ± 0.05 |
a
Abbreviations: Ade, adenine; Thi, thiamine; Pan, pantothenate.
b
TPP levels are expressed as picomoles per milligram (dry weight) of cells (29), and CoA levels are expressed as nanomoles per milligram (dry weight) of cells (1, 15). Data are averages of three independent experiments. NA, not applicable.
c
Specific growth rates were determined as μ = ln(X/Xo)/T, as previously described (24). Growth curves were performed with three independent innocula, and the rates were averaged.