TABLE 3.
panB628(Ts) generates a temperature-conditional thiamine auxotrophy
| Temp (°C) | Specific growth rate (μ) on minimal medium witha:
|
||
|---|---|---|---|
| Ade | Ade-Thi | Ade-Pan | |
| 30 | 0.34 | 0.45 | 0.46 |
| 32 | 0.12 | 0.32 | 0.50 |
| 35 | 0.07 | 0.12 | 0.42 |
| 37 | 0.03 | 0.13 | 0.50 |
a
Medium contained gluconate (22 mM) as the sole carbon and energy source plus the indicated additions. Data are for growth of strain DM4961 [purF2085 panE628(Ts)] at the indicated temperatures and are from a representative experiment. Growth curves were performed as described previously (24) with the specific growth rate determined as μ = ln (X/Xo)/T. Abbreviations: Ade, adenine; Thi, thiamine; Pan, pantothenate.