FIGURE 5.

Assessment of functional EC₅₀ values of NKG2A blocking antibodies monalizumab and Z199. NKG2A/CD94 expressing reporter cells were stimulated with control K562S, K562S‐HLA‐E^HLA‐G, K562S‐HLA‐E^HLA‐B8, K562S‐HLA‐E^HLA‐A2 and K562S‐HLA‐E^HLA‐C4 for 24 h in the presence of monalizumab (a) and Z199 (b) at concentrations ranging from 31.6 μg/ml to 10 ng/ml. White bars represent NKG2A inhibition in the absence of an antibody. Results are depicted of three (monalizumab) and two (Z199) independent experiments performed in duplicate. Left panels; data are normalized to control K562S cells in the absence or presence of the indicated antibody concentrations. Right panels; inhibition curves and half‐maximum effective concentrations (EC₅₀) were calculated for the NKG2A antibodies from normalized data.