Figure 6.

Cell viability studies and cellular uptake of PEGylated graphene oxide. (A) CPI444 and vatalanib cell cytotoxicity were calculated in GBM U87 cells. Cells were treated with drugs individually along with different concentrations for 48 h. After 48 h, IC₅₀ values were calculated. (B) Cytotoxicity analyses of GO and GO-PEG were performed in GBM U87 cells. The PEGylated graphene oxide carrier did not show significant cytotoxicity in the GBM cell line up to 20 µg/ml. (C) In the GBM U87 cell line IC₅₀ value of CPI444 + vatalanib@GO-PEG is ~14 µM. (D) To confirm the cellular internalization of PEGylated GO nanoparticles in the GBM cells, we conducted a FACS study after 24 h of incubation with different concentrations of PEGylated graphene oxide nanoparticles; MFI (Mean Fluorescence Intensity) in arbitrary units (AU); ****p <0.0001 and ***p <0.001.