Figure 6.
Genic profile of several proteins in the vessel wall of Old Obese subjects (n=5) before and after incubation with SRT1720. A, Expression of p66Shc. B, Expression of Arginase II. C, Expression of Sirt3. Notably, the ChIP assay did not detect the binding of SIRT1 (silent information regulator 1) on the Sirt3 promoter. D, qPCR after ChIP assay showing binding of SIRT1 on the promoter region of p66Shc before and after incubation with SRT1720. E, qPCR after ChIP assay showing binding of SIRT1 on the promoter region of Arginase II before and after incubation with SRT1720. F–I Expression of mitochondria respiratory chain enzymes: ATP synthase 6 (F), Cytochrome b (G), NADH dehydrogenase 2 (H) and NADH dehydrogenase 5 (I). Data are presented as mean±SD and were compared by the Kruskal-Wallis test with Dwass-Steel-Critchlow-Flinger post hoc test. A P<0.05 was considered significant. **P<0.01. ArgII indicates arginase II; ATP6, ATP synthase 6; Cytb, Cytochrome b; ND2, NADH dehydrogenase 2; OO, old obese; and YN, young nonobese.