Skip to main content
. 2022 Aug 26;9(4):ENEURO.0089-22.2022. doi: 10.1523/ENEURO.0089-22.2022

Figure 2.

Figure 2.

Whisker asymmetry and eye position in relation to the cued direction of movement. A, Averages of whisker positions. Median positions for the left whisker (thick red line) and right whisker (thick blue line) show that whisker asymmetry was related to the direction of movement, and was evident early in the backward movement of mice out of the lane. The shaded areas show the 25th to 75th percentiles. The asymmetry between whiskers emerges at the beginning of backward movement, and reverses once during the course of the turn and a second time during the forward motion. Asymmetry vanishes when mice wait for and expect reward. Note that whisker asymmetry on right-turn and left-turn trials are almost mirror images of each other. B, Averages of eye positions. Median positions for the left eye (thick red line) and the right eye (thick blue line) move conjugately in the behavioral state-specific manner. The shaded areas show the 25th to 75th percentiles. Note that the average eye positions on right-turn and left-turn trials are inverted images of each other. Whisker and eye positions were normalized for amplitude (see Materials and Methods) and for time (because trial durations varied; see Materials and Methods). The numbers of behavioral epochs used were as follows: At End, n = 54 (left-turning) epochs and n = 36 (right-turning) epochs; Backward, n = 54 (left-turning) epochs and n = 36 (right-turning) epochs; Turn, n = 52 (left-turning) epochs and n = 40 (right-turning) epochs; Forward, n = 63 (left-turning) epochs and n = 50 (right-turning) epochs; Expect, n = 51 (left-turning) epochs and n = 38 (right-turning) epochs; Lick, n = 51 (left-turning) epochs and n = 36 (right-turning) epochs. C, D, Tukey box plots of average whisker asymmetry (C) and eye deviation (D) during different behavioral epochs. p < 0.0001, Kruskal–Wallis test. Post hoc pairwise tests were performed to compare with the first half of the end-of-lane epoch, using Dunn’s test with Bonferroni’s correction. ***p < 0.001, **p < 0.01, *p < 0.05, NS, p > 0.05.