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. Author manuscript; available in PMC: 2022 Aug 30.
Published in final edited form as: Biochem Biophys Res Commun. 2017 May 17;488(3):547–553. doi: 10.1016/j.bbrc.2017.05.084

Fig. 2.

Fig. 2.

Interaction between GIR1 or GIR2 and GL2. (A) GIR1 and GIR2 interact with GL2 in the yeast two-hybrid system. The indicated dilutions of yeast cells were grown in the absence or presence of histidine as shown. Protein interaction was detected as cell growth on histidine-deficient medium and as β-galactosidase (β-gal) activity on a nitrocellulose filter. (B) GIR1 and GIR2 interact with GL2 in planta, and the interacting proteins accumulate in the cell nucleus. Protein interaction was detected by BiFC. YFP signal is in yellow. Fluorescence images are single confocal sections. Scale bars = 20 μm.