Figure 6. Snc1 recycling is affected in sso2 mutants.
(A–D) Representative images of sso1Δ SSO2 control, sso1Δ sso2M5 sso1Δ sso2M6, and sso1Δ sso2M7 mutant cells. Strains harboring a CEN plasmid expressing GFP-Snc1 were grown in SC-Ura medium overnight at 25°C reaching an OD600 around 0.5. Cells were further diluted and shifted to 37°C for 90 min prior to imaging. GFP-Snc1 localized predominantly to the PM in SSO2 control cells and sso2M6 cells yet formed GFP patches (indicated by arrows) near small buds or bud necks in sso2M5 and sso2M7 mutant cells. Scale bar, 5 µm. (E) Quantitation of cells with GFP-Snc1 patches. GFP-Snc1 patches near bud tips or necks were scored. Error bar represents SD, n=3. *p<0.05, **p<0.005.