Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Aug 30;56(6):854–873. doi: 10.1134/S0026893322060103

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© Pleiades Publishing, Inc. 2022, ISSN 0026-8933, Molecular Biology, 2022, Vol. 56, No. 6, pp. 854–873. © Pleiades Publishing, Inc., 2022.ISSN 0026-8933, Molecular Biology, 2022. © Pleiades Publishing, Inc., 2022.

This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.

PMC Copyright notice

Fig. 1. — Variety of programmable nucleases. (a–d) Endonucleases that do not require a PAM sequence in the target for catalytic activity; (e, f) endonucleases that require the presence of PAM (protospacer adjacent motif) in the 5'-region from the recognition site; (g, h) endonucleases that require the presence of PAM in the 3'-region from the recognition site. (a) Chimeric endonuclease based on the zinc finger DNA-binding domain (PDB ID: 7ZNF) and FokI (PDB ID: 2FOK) (protein structures are combined for illustration); (b) chimeric endonuclease based on the TALE DNA-binding domain (PDB ID: 4HPZ) and FokI (PDB ID: 2FOK) (protein structures are combined for illustration); (c) meganuclease I-AabMI (PDB ID: 4YIT); (d) Argonaute protein can be an RNA- or DNA-guided endonuclease and cleave RNA or DNA (TtAgo, PDB ID: 4NCB); (e) RNA-dependent DNA-endonuclease TnpB (TnpB ISDra2 structure was predicted using the AlphaFold2 algorithm [1]); (f) RNA-dependent DNA-endonuclease Cas12 (PDB ID: 7EU9) [2]; (g) RNA-dependent DNA-endonuclease Cas9 (PDB ID: 6M0X) [3]; and (h) RNA-dependent DNA-endonuclease IscB (the structure was predicted using the AlphaFold2 algorithm [1]).