Skip to main content
. 2022 Aug 23;2022:8035083. doi: 10.1155/2022/8035083

Figure 3.

Figure 3

circKIF4A acts as a sponge for miR-335. (a) The nucleocytoplasmic fraction assay revealed that circKIF4A is mainly localized in the cytoplasm, of BC cells. GAPDH was used as a cytoplasmic control and 18 S as a nuclear control. (b) Binding sites predicted for miR-335 in the circKIF4A sequence. (c) A dual luciferase reporter assay showed circKIF4A interacted with miR-335. (d) Based on RNA immunoprecipitation (RIP) assays, miR-335 was remarkably elevated in the MS2-circKIF4A-wt group. (e) Transfecting si-circKIF4A induced an increase in miR-335 expression. ∗∗P < 0.01.