TABLE 1.
Substance with which cells were grown aerobically | CoA ligase activity (nmol min−1 mg of protein−1) with substrate:
|
||
---|---|---|---|
PA | 4-OHPA | B | |
PA | 153 | 18 | 37 |
4-OHPA | 17 | 23 | 7 |
B | 6 | 4 | 196 |
Phe | 194 | 20 | 34 |
PA (anaerobic) | 139 | 21 | 106 |
Adipate | 5 | 4 | NDb |
Acetate | 4 | 3 | ND |
The cell extracts were the supernatant after centrifugation at 100,000 × g. Enzyme activities were determined by a coupled enzyme-spectrophotometric assay.
ND, not determined.