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. 2000 Jan;182(2):286–294. doi: 10.1128/jb.182.2.286-294.2000

TABLE 1.

CoA-ligase activities in a protein fraction of A. evansii cell extracts precipitated at 60% ammonium sulfatea

Substance with which cells were grown aerobically CoA ligase activity (nmol min−1 mg of protein−1) with substrate:
PA 4-OHPA B
PA 153 18 37
4-OHPA 17 23 7
B 6 4 196
Phe 194 20 34
PA (anaerobic) 139 21 106
Adipate 5 4 NDb
Acetate 4 3 ND
a

The cell extracts were the supernatant after centrifugation at 100,000 × g. Enzyme activities were determined by a coupled enzyme-spectrophotometric assay. 

b

ND, not determined.