Table 1.
Samples used to evaluate specificity during the standardization of the nested PCR assay for the diagnosis of Paracoccidioides brasiliensis.
| Kind of patient | Type of sample | Diagnosis | ||
|---|---|---|---|---|
| Negative controls (n:51) | Respiratory symptomatic (n:30) | BAL (25) | ||
| BL (4) | ||||
| Sputum (1) | ||||
| Healthy individuals (n:21) | Whole blood (21) | |||
| Positive controls (n:25) | Respiratory symptomatic (n:25) | BAL (2) | Paracoccidioides brasiliensis (n:25) | |
| Biopsies (15) | ||||
| Sputum (8) | ||||
| Clinical samples (n:191) | Samples used to evaluate the specificity (n:115) | Respiratory symptomatic | Body fluids (4) | Aspergillus fumigatus (n:1) |
| Candida albicans (n:1) | ||||
| Candida tropicalis (n:2) | ||||
| Tracheal aspirates (9) | Aspergillus fumigatus (n:1) | |||
| Candida albicans (n:6) | ||||
| Candida tropicalis (n:1) | ||||
| Mycobacterium tuberculosis (n:1) | ||||
| CSF (24) | Cryptococcus neoformans (n:24) | |||
| Biopsies (22) | Aspergillus fumigatus (n:4) | |||
| Aspergillus flavus (n:2) | ||||
| Candida spp (n:1) | ||||
| Candida albicans (n:3) | ||||
| Candida parasilopsis (n:1) | ||||
| Candida guillermondii (n:1) | ||||
| Cryptococcus neoformans (4) | ||||
| Histoplasma capsulatum (n:5) | ||||
| Mycobacterium tuberculosis (n:1) | ||||
| Sputum (9) | Aspergillus fumigatus (n:2) | |||
| Mycobacterium tuberculosis (n:7) | ||||
| BL (16) | Aspergillus fumigatus (n:3) | |||
| Mycobacterium tuberculosis (n:7) | ||||
| Cryptococcus neoformans (6) | ||||
| BAL (31) | Aspergillus fumigatus (n:4) | |||
| Aspergillus flavus (n:1) | ||||
| Aspergillus versicolor (n:1) | ||||
| Mycobacterium tuberculosis (n:4) | ||||
| Cryptococcus neoformans (10) | ||||
| Histoplasma capsulatum (n:5) | ||||
| Pneumocystis jirovecii (n:6) | ||||
BAL, bronco alveolar lavage; BL, bronchial lavage; CSF, cerebrospinal fluid.