Fig. 2.

Effect of iron deprivation on cell surface properties. (a) Relative sedimentation of M. smegmatis cells under iron deprivation. Left panel shows OD₅₉₀ of untreated (control), 2,2 BP (35 μg/ml) and siderophore mutant Δ011-14 cells depicted on Y-axis with respect to time noted at 3 and 22 h on X-axis. Right panel depicts sedimentation rates per hour on Y-axis of 2,2 BP (35 μg/ml) and mutant Δ011-14 cells with respect to control on X-axis, calculated by estimating the difference in OD₅₉₀ from 0 till 22 h per unit time interval. Mean of three independent sets of experiments ± SD (shown by error bars) are depicted. (b) Altered colony morphology under iron deprivation. Colony morphology of control, iron deprived (2,2 BP) and mutant Δ011-14 cells were observed on 7H10-based medium. Corresponding area of colony borders were focused (10×-magnification) and one of the colony border depicted by an arrow is enlarged and shown in the inset. (c) Thin layer chromatogram showing altered total lipid profile under iron deprivation.