Fig. 4.

Effect of iron deprivation on Mycobacterium cell adhesion. Iron deprivation (2,2 BP (35 μg/ml) and siderophore mutant Δ011-14) inhibits the cell adhesion of Mycobacterium smegmatis to microtiter polystyrene surface in presence of three known anti-TB drugs, i.e. INH, EMB and RIF (1 μg/ml, 0.0625 μg/ml, 0.0625 μg/ml). Reduction in growth percent is quantified by using crystal violet dye and depicted as bar graph. Mean of OD₆₀₀ ± SD of three independent sets of experiments are depicted on Y-axis and * depicts p value <0.05.